Invention Grant
- Patent Title: Method for detecting variation of gene for non-diagnostic purpose based on fluorescence quenching and probe thereof
- Patent Title (中): 基于荧光猝灭及其探针检测非诊断目的基因变异的方法
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Application No.: US14352438Application Date: 2012-10-17
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Publication No.: US09574227B2Publication Date: 2017-02-21
- Inventor: Rui Shi , Bochun Huang
- Applicant: SHANGHAI QY BIOTECH CO., LTD.
- Applicant Address: CN Shanghai
- Assignee: SHANGHAI QY BIOTECH CP., LTD.
- Current Assignee: SHANGHAI QY BIOTECH CP., LTD.
- Current Assignee Address: CN Shanghai
- Priority: CN201110318975 20111019
- International Application: PCT/CN2012/083104 WO 20121017
- International Announcement: WO2013/056651 WO 20130425
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12Q1/68
Abstract:
A method for detecting variation of gene based on fluorescence quenching quantification comprises: performing single base extension at a specific site of a gene to be detected with marked probes and marked dideoxyribonucleotide triphosphate; detecting fluorescence quenching values, and determining SNP of the gene to be detected. The present invention also provides an oligonucleotide probe for the method.
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