Invention Grant
US09574233B2 Method for detecting the synthesis and/or amplification of a target nucleic acid sequence 有权
检测靶核酸序列的合成和/或扩增的方法

  • Patent Title: Method for detecting the synthesis and/or amplification of a target nucleic acid sequence
  • Patent Title (中): 检测靶核酸序列的合成和/或扩增的方法
  • Application No.: US14361049
    Application Date: 2012-11-29
  • Publication No.: US09574233B2
    Publication Date: 2017-02-21
  • Inventor: Maria Elena TurbaElisa Zambon
  • Applicant: GENEFAST S.R.L.
  • Applicant Address: IT Bazzano (BO)
  • Assignee: Genefast S.R.L.
  • Current Assignee: Genefast S.R.L.
  • Current Assignee Address: IT Bazzano (BO)
  • Agency: RatnerPrestia
  • Priority: ITMI2011A2177 20111129
  • International Application: PCT/IB2012/056825 WO 20121129
  • International Announcement: WO2013/080154 WO 20130606
  • Main IPC: C12P19/34
  • IPC: C12P19/34 C12Q1/68 C12Q1/70
Method for detecting the synthesis and/or amplification of a target nucleic acid sequence
Abstract:
The present invention relates to a method for the detection of nucleic acid synthesis and/or amplification, characterized in that the method includes adding at least one colorimetric metal indicator and at least one bland magnesium chelator to a reaction mixture for nucleic acid amplification. The present invention further relates to a kit for carrying out such a method and to the use thereof in the health, food and agricultural or veterinary fields.
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