Invention Grant
- Patent Title: Method for detecting a target particle
- Patent Title (中): 检测目标粒子的方法
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Application No.: US14465208Application Date: 2014-08-21
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Publication No.: US09575060B2Publication Date: 2017-02-21
- Inventor: Kazutaka Nishikawa , Takuya Hanashi
- Applicant: OLYMPUS CORPORATION
- Applicant Address: JP Tokyo
- Assignee: OLYMPUS CORPORATION
- Current Assignee: OLYMPUS CORPORATION
- Current Assignee Address: JP Tokyo
- Agency: Westerman, Hattori, Daniels & Adrian, LLP
- Priority: JP2012-095101 20120418
- Main IPC: G01N21/64
- IPC: G01N21/64 ; G01N21/76 ; G01N33/52 ; G01N33/53 ; G02B21/00 ; G01N33/543 ; G01N15/14

Abstract:
Provided is a method for detecting a target particle that is a method for detecting a non-luminescent target particle dispersed and randomly moving in a sample solution using an optical system of a confocal microscope or multi-photon microscope, having: (a) preparing a sample solution containing target particles, and labeling particles of which the average outer diameter is less than 15% of the diameter of a photodetection region of the optical system, binding two or more molecules of the labeling particles per molecule of the target particles in the sample solution, and forming a non-luminescent complex of which the outer diameter is 15% or more of the diameter of the photodetection region; and, (b) calculating the number of molecules of the complex in the sample solution prepared in the (a) using an inverse scanning molecule counting method.
Public/Granted literature
- US09547002B2 Method for detecting a target particle Public/Granted day:2017-01-17
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