A bright dot detection method adapted to be used in a display panel including a plurality of gate lines, a plurality of source lines and a bright dot detection module. The plurality of gate lines and the plurality of sources line are interlaced thereby forming a plurality of pixels. The bright dot detection method includes: driving the plurality of pixels through enabling the plurality of gate lines simultaneously, thereby forming a first bright line in a first direction; and driving the plurality of pixels through enabling the plurality of gate lines sequentially and providing a control signal to the bright dot detection module, thereby forming a second bright line in a second direction, wherein a bright dot is positioned where the first and second bright lines meet with each other. A display panel is also disclosed.