A non-display signal encoding method cooperates with a matrix substrate including a plurality of electrodes. The electrodes cross each other and transmit a plurality of display signals and a plurality of non-display signals. The non-display signal encoding method comprises steps of: transmitting the non-display signals by at least one of the electrodes during a first time; and receiving by coupling at least a part of the non-display signals by at least an external object, wherein the non-display signals have at least three states, i.e. a first state, a second state, a third state or their any combination, and the first, second and third states are different from one another. A matrix substrate is also disclosed.