The present invention generally relates to the fields of parasitology and immunology, and especially to a vaccine against Rhipicephalus ticks. In particular the invention relates to a composition comprising a first and a second protein, in particular Bm86 and Subolesin proteins, including their homologs or immunogenic fragments; to the use of such a composition as a vaccine against Rhipicephalus ticks, and to the use of the first and the second isolated proteins for the vaccination of a target against Rhipicephalus ticks.