A harness routing structure for a saddled vehicle includes a side body cover section, a center cover, a fuel tank having a fuel filler opening at an upper surface, and a refueling tray configured to be supported by the fuel tank, in the harness routing structure for a saddled vehicle used in the saddled vehicle having a straddling space disposed at an upper side of the center cover and through which a passenger passes his/her legs to ride the vehicle, a main harness extending from a front side of the straddling space to a rear side of the straddling space is routed below the center cover, is passed through a side of the refueling tray, and is routed between the side body cover section and the refueling tray.