Invention Grant
US09581601B2 Method of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonic acid as derivatization reagent
有权
通过使用新的衍生化试剂和合成5-甲酰基 - 苯-1,3-二磺酸作为衍生试剂，检测氨基酸序列和/或肽和蛋白质的鉴定方法
- Patent Title: Method of detection of amino acid sequence and/or identification of peptides and proteins, by use of a new derivatization reagent and synthesis of 5-formyl-benzene-1,3-disulphonic acid as derivatization reagent
- Patent Title (中): 通过使用新的衍生化试剂和合成5-甲酰基 - 苯-1,3-二磺酸作为衍生试剂，检测氨基酸序列和/或肽和蛋白质的鉴定方法
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Application No.: US14117395Application Date: 2011-05-18
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Publication No.: US09581601B2Publication Date: 2017-02-28
- Inventor: Mario Cindric , Zdenko Hamersak , Ivana Dodig
- Applicant: Mario Cindric , Zdenko Hamersak , Ivana Dodig
- Applicant Address: HR Zagreb
- Assignee: Rudjer Boskovic Institute
- Current Assignee: Rudjer Boskovic Institute
- Current Assignee Address: HR Zagreb
- Agency: Nixon Peabody LLP
- Agent Jeffrey L. Costellia
- International Application: PCT/HR2011/000019 WO 20110518
- International Announcement: WO2012/156764 WO 20121122
- Main IPC: G01N33/68
- IPC: G01N33/68 ; C07C303/02 ; C07C303/22 ; C07C333/04
Abstract:
Present invention refers to a novel and improved method of derivatization and detection of amino acid sequence and/or identification of proteins, peptides by a new derivatization compound. Precisely, the method discloses a novel approach to derivatization of peptides or proteins by compounds comprising two or more sulfonyl groups and analysis of derivatized analytes in negative mode of operation of mass spectrometer. This method allows unambiguous analysis of amino acid sequence of long-chain peptides/proteins. Also, the invention discloses a novel synthesis procedure of 5-formyl-benzene-1,3-disulphonic acid as derivatization compound.
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