A preparation method of an antibacterial cornea repair material includes (1) purifying type I collagen extracted from beef tendons, and preparing a collagen solution; (2) casting the collagen solution into a mold to form a membrane by air drying at room temperature; (3) soaking the membrane in an antibiotic solution, stirring until the membrane is in contact with the antibiotic solution, adding a crosslinking agent and a catalyst to the antibiotic solution contacted with the membrane, and stirring the antibiotic solution contacted with the membrane with the crosslinking agent and the catalyst added therein to perform a crosslinking reaction to obtain a crosslinked membrane; and (4) washing the crosslinked membrane material with deionized water, and air drying at room temperature. The antibacterial cornea repair material has relatively good mechanical properties, optical properties, biocompatibility, and antibacterial effect, and can be used for repairing and replacing damaged cornea tissue.