The present invention provides a system and method for generating a dataset of uncompressed color images taken with three digital cameras and exhibiting different levels of real noise, resulting from low-light conditions. For each scene there are, on average, two low-noise and two noisy images that are aligned at the pixel level both spatially and in intensity. In one embodiment, the dataset contains over 100 scenes and more than 400 images, including both 16-bit RAW formatted images and 8-bit BMP pixel and intensity aligned images from three digital cameras. The generated dataset of real noise images is useful in the evaluation of denoising algorithms.