Provided is a matrix type antimicrobial vehicle, including: a matrix layer comprising an antimicrobial agent and an adhesive; an antimicrobial agent-releasing layer formed on one surface of the matrix layer; and a release liner formed on the other surface of the matrix layer, and a method for preparing the same. The matrix type antimicrobial vehicle is incorporated into a package having a medical kit so that the antimicrobial agent is released to the surface of medical kit or package continuously. Therefore, it is possible for the surface of the medical kit or package to maintain a constant concentration of antimicrobial agent continuously even when the medical kit or package is stored for a long time.