Invention Grant
US09593307B2 Defined media for expansion and maintenance of pluripotent stem cells
有权
定义用于扩增和维持多能干细胞的培养基
- Patent Title: Defined media for expansion and maintenance of pluripotent stem cells
- Patent Title (中): 定义用于扩增和维持多能干细胞的培养基
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Application No.: US15150349Application Date: 2016-05-09
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Publication No.: US09593307B2Publication Date: 2017-03-14
- Inventor: Alireza Rezania
- Applicant: Janssen Biotech, Inc.
- Applicant Address: US PA Horsham
- Assignee: Janssen Biotech, Inc.
- Current Assignee: Janssen Biotech, Inc.
- Current Assignee Address: US PA Horsham
- Agent Lois A. Gianneschi
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C12N5/02 ; C12N5/0735
Abstract:
The present invention provides methods to promote the proliferation of undifferentiated pluripotent stem cells in defined media. Specifically, the invention provides a defined cell culture formulation for the culture, maintenance, and expansion of pluripotent stem cells, wherein culturing stem cells in the defined cell culture formulation maintains the pluripotency and karyotypic stability of the cells for at least 10 passages. Further disclosed is a cell population, grown under defined media conditions, that expresses OCT4, SOX2, NANOG, and FOXA2.
Public/Granted literature
- US20160251615A1 DEFINED MEDIA FOR EXPANSION AND MAINTENANCE OF PLURIPOTENT STEM CELLS Public/Granted day:2016-09-01
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