Methods, apparatus, systems and articles of manufacture detect spoofing attacks for video-based authentication are disclosed. Disclosed example method to perform video-based authentication include determining whether a sequence of input images provided to perform video-based authentication of a subject exhibits a first region having fluctuating pixel values. Such example methods also include determining that the sequence of input images is associated with a spoofing attack in response to determining that the sequence of input images exhibits the first region having fluctuating pixel values.