Invention Grant
- Patent Title: Cyprinid herpesvirus II-sensitive brain tissue cell line of Carassius auratus gibelio and establishing method and use thereof
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Application No.: US14774041Application Date: 2014-12-03
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Publication No.: US09598671B2Publication Date: 2017-03-21
- Inventor: Jie Ma , Lingbing Zeng , Nan Jiang , Qian Chen , Hui Zhao
- Applicant: Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science
- Applicant Address: CN Wuhan, Hubei
- Assignee: YANGTZE RIVER FISHERIES RESEARCH INSTITUTE, CHINESE ACADEMY OF FISHERY SCIENCES
- Current Assignee: YANGTZE RIVER FISHERIES RESEARCH INSTITUTE, CHINESE ACADEMY OF FISHERY SCIENCES
- Current Assignee Address: CN Wuhan, Hubei
- Agency: Muncy, Geissler, Olds & Lowe, P.C.
- Priority: CN201310658939 20131209
- International Application: PCT/CN2014/092954 WO 20141203
- International Announcement: WO2015/085886 WO 20150618
- Main IPC: C12N5/079
- IPC: C12N5/079 ; A61K39/245 ; C12Q1/70 ; G01N33/50 ; C12N7/00
Abstract:
The present invention discloses a cyprinid herpesvirus II-sensitive brain tissue cell line of Carassius auratus gibelio and establishing method and use thereof. The cell line is deposited in China Center for Type Culture Collection under an accession number of CCTCC No: C2013179. The brain tissue cell line of Carassius auratus gibelio is in good growth state and sensitive to CyHV-2 that is presently hardly cultured with ordinary fish cell lines. After six passages of CyHV-2 in GiCB cells, viral nucleic acid can still be detected and a cytopathic effect is stable. When ultrathin microscopic sections are prepared from cells having the cytopathic effect, considerable mature CyHV-2 virions and their replication process can be observed in GiCB cells. The construction method of the brain tissue cell line of Carassius auratus gibelio of the present invention has high repeatability, scientific and reasonable conditions.
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