In a diaphragm pump having a fluorine resin-made rolling diaphragm including: an inner circumferential portion that elongates along an outer circumferential surface of a piston; an outer circumferential portion that elongates along the inner circumferential surface of a cylinder; and a folded portion that is formed by folding back in a tubular space between the cylinder and the piston; and a pump chamber is separated by the rolling diaphragm in the cylinder, the rolling diaphragm is produced in an extended attitude corresponding to an attitude obtained when, by a projective movement of the piston, the outer circumferential portion is eliminated, and the folding of the folded portion is opened.