An image sensing apparatus includes an image sensor including a plurality of pixels and configured to sense an optical image formed from fluorescence or phosphorescence generated by excitation light from an excitation light source when periods during which pixels perform the photoelectric conversion sequentially end on the respective rows, a mechanical shutter configured to control exposure on the image sensor, and a control unit configured to control the image sensor and the mechanical shutter so as to prevent overlapping between an excitation period and a detection period, the excitation period being a period during which the excitation light source emits excitation light, the detection period being a period during which the plurality of pixels are commonly set in a state of performing the photoelectric conversion for detecting fluorescence or phosphorescence and the mechanical shutter is open.