The invention provides a conjugated compound containing a bis(phenylsulfonyl)benzene structure, a preparation method and an application thereof, the compound includes a chemical structure with one of structural general formulas as follows: the invention obtains a conjugated compound containing a bis(phenylsulfonyl)benzene structure by selecting multiple kinds of conjugated aromatic units to react with halogen-substituted bis(phenylsulfonyl)benzene in the manner of Suzuki coupling reaction, Buchwald-Hartwig coupling reaction or copper-catalyzed halogenated aromatic amination reaction. The novel compound prepared by the invention has the characteristic of fluorescence as well as a certain electrical conductivity, and thus can be applied to fabricate a light-emitting layer or an electron transport layer of an organic electroluminescent diode.