Invention Grant
- Patent Title: In vitro reconstituted plant virus capsids for delivering RNA genes to mammalian cells
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Application No.: US14540816Application Date: 2014-11-13
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Publication No.: US09605031B2Publication Date: 2017-03-28
- Inventor: William M. Gelbart , Charles M. Knobler , Rees F. Garmann , Odisse Azizgolshani , Ruben D. Cadena-Nava
- Applicant: The Regents of the University of California
- Applicant Address: US CA Oakland
- Assignee: The Regents of the University of California
- Current Assignee: The Regents of the University of California
- Current Assignee Address: US CA Oakland
- Agency: Gates & Cooper LLP
- Main IPC: C07K14/005
- IPC: C07K14/005 ; C12N7/04 ; C12N5/00

Abstract:
The invention provides compositions of matter comprising a cowpea chlorotic mottle virus capsid protein (CCMV CP) and a ribonucleic acid, as well as methods for using such compositions. In such compositions, the cowpea chlorotic mottle virus capsid protein envelops the ribonucleic acid so as to for a capsid that can inhibit the degradation of the ribonucleic acid (e.g. by RNAses). A method of delivering a ribonucleic acid into the cytoplasm of a mammalian cell is also provided. Typically, the method comprises the steps of combining the mammalian cell with a composition of matter described herein under conditions selected to allow the cowpea chlorotic mottle virus capsid to contact the mammalian cell and deliver the ribonucleic acid into the cytoplasm of a mammalian cell.
Public/Granted literature
- US20150140663A1 IN VITRO RECONSTITUTED PLANT VIRUS CAPSIDS FOR DELIVERING RNA GENES TO MAMMALIAN CELLS Public/Granted day:2015-05-21
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