A diaphragm pump includes a first plate, a second plate and a diaphragm having first and second side surfaces. A fluid chamber is defined by the first plate and the first side of the diaphragm, a fluid inlet fluidly connected to the fluid chamber and a fluid outlet fluidly connected to the fluid chamber. An air chamber is defined by the second plate and the second side of the diaphragm, such that the diaphragm is retained between the first plate and the second plate. A clamp moves between a first position in which the first plate is fixedly connected to the second plate and the diaphragm, and a second position in which the first plate is disconnected from the second plate and the diaphragm to permit disassembly of the first plate, the second plate and the diaphragm.