A fabrication method of a substrate, relates to a field of a display technology, which can avoid a deviation between a line width of a black matrix or a color filter layer actually fabricated and a preset line width, so that the black matrix can just completely shield thin film transistor, a data line and a gate line, and meanwhile the black matrix or the color filter layer more refined are obtained, which improves the display effect of the liquid crystal display. The fabrication method of the substrate comprises: forming a to-be-treated layer, forming a light-shielding layer on the to-be-treated layer, and forming a pattern of the light-shielding layer by a patterning process, wherein the light-shielding layer is made of metal; performing a patterning process on the to-be-treated layer by using the pattern of the light-shielding layer as a mask; and removing the light-shielding layer.