The invention discloses a backlight driving method, a backlight driving device, and a display device. The method comprises steps of: acquiring turn-on timings of respective areas of a backlight source of a display device and an opening timing of a 3D glasses; adjusting driving signals for the respective areas of the backlight source based on each length of overlapping time between the opening timing of the 3D glasses and each of the turn-on timings of the respective areas of the backlight source, thus making brightness of light from pictures corresponding to the respective areas of the backlight source in the display device be the same, said light being received through the 3D glasses; and driving the corresponding areas in the backlight source to be turned on by using the adjusted driving signals for the respective areas of the backlight source.