The invention provides a pixel unit driving circuit, a display substrate, a display panel and a display device. The pixel unit driving circuit is used for driving a plurality of pixel units, wherein each of the pixel units comprises sub-pixels of different colors. The pixel unit driving circuit comprises a plurality of driving powers corresponding to the sub-pixel of different colors, wherein the sub-pixels of a same color are connected with a same driving power, and each of the driving powers is used for supplying a corresponding driving voltage to the sub-pixels of a color corresponding to the driving power.