A fluid line treatment kit for a dairy farm milking system having a vacuum subsystem for imparting vacuum within a fluid line is provided. The fluid line treatment kit includes at least one ozone gas source; a conduit associated with each ozone gas source configured to convey ozone gas to within the fluid line at a respective location; and a control system configured to trigger each ozone gas source to produce ozone gas while the vacuum subsystem is actuated. Vacuum imparted by the vacuum subsystem in the fluid line draws ozone gas via the at least one conduit into and through the fluid line.