Invention Grant
- Patent Title: Method for manufacturing multilayered cell sheet, multilayered cell sheet having vascular network obtained thereby, and method of use thereof
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Application No.: US13823007Application Date: 2011-09-14
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Publication No.: US09617519B2Publication Date: 2017-04-11
- Inventor: Katsuhisa Sakaguchi , Tatsuya Shimizu , Hidekazu Sekine , Mitsuo Umezu , Teruo Okano
- Applicant: Katsuhisa Sakaguchi , Tatsuya Shimizu , Hidekazu Sekine , Mitsuo Umezu , Teruo Okano
- Applicant Address: JP Tokyo
- Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
- Current Assignee: TOKYO WOMEN'S MEDICAL UNIVERSITY
- Current Assignee Address: JP Tokyo
- Agency: Kilpatrick Townsend & Stockton LLP
- Priority: JP2010-225201 20100914
- International Application: PCT/JP2011/071056 WO 20110914
- International Announcement: WO2012/036225 WO 20120322
- Main IPC: C12N5/071
- IPC: C12N5/071 ; A61F2/02 ; C12M3/00 ; C12M1/12 ; C12N5/077
Abstract:
A method for manufacturing a multilayered cell sheet characterized in fabricating a vascular bed that constructs a vascular network extending to the surface from a channel for perfusing a medium, the channel being embedded in a gel; and layering a cell sheet onto the vascular bed to construct a vascular network in the cell sheet. This manufacturing method makes it possible to construct a vascular network in the cell sheet and to fabricate a thick multilayered cell sheet in a simple manner by layering cell sheets. Such a thick multilayered cell sheet is useful as an in-vivo tissue substitute in regenerative medicine involving a variety of tissues.
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