A display substrate includes a gate metal pattern comprising a gate line extending in a first direction and a gate electrode electrically connected to the gate line, a data metal pattern comprising a data line extending in a second direction crossing the first direction, a source electrode electrically connected to the data line and a drain electrode spaced apart from the source electrode, an organic layer disposed on the data metal pattern, a repair hole penetrating the organic layer and exposing a crossing area in which the gate line crosses with the data line and a pixel electrode disposed on the organic layer and electrically connected to the drain electrode.