The present disclosure provides a display substrate and a mask plate, the display substrate comprising a plurality of sub display substrates, each of the sub display substrates comprising a plurality of pixel units, each pixel unit comprising a pixel electrode, a common electrode and a source-drain channel, wherein, from the center of the display substrate to the edge of the display substrate, the plurality of sub display substrates are arranged from large to small according to the overlapping area of the pixel electrode and the common electrode and/or the plurality of sub display substrates are arranged from small to large according to the width to length ratio of the source-drain channel of the sub display substrate. The present disclosure can avoid electrical badness of the sub display substrates located at the edges.