The present invention provide a display substrate, a fabricating method thereof, and a display apparatus, and belongs to the field of display technology. The display substrate comprises a plurality of pixels, each of the pixels is divided into a plurality of light emitting units, each of the light emitting units comprises an anode, a cathode, a carrier transport layer, and a light emitting layer, wherein at least one of the plurality of light emitting units comprises a light emitting layer and at least one process auxiliary layer; the process auxiliary layer and the light emitting layer in other light emitting units are formed into an integral portion from a same material in a film forming process. Display resolution can be improved in the present invention.