Systems and methods for calibrating scanning devices are described. The method comprises dividing a dark scan image of a calibration strip into one or more regions. The dark scan image is further analyzed to obtain at least one data point from each of the one or more regions. Further, calibration values for each of the one or more regions are computed based on the at least one data point for calibrating the scanning device. The calibration values include mean and standard deviation of the data points for each of the one or more regions.