This disclosure provides systems, methods and apparatus for multi-channel satellite calibration. In one aspect, a calibration system can provide a transmit calibration signal based on a reference calibration signal to a satellite. The satellite can transmit back a receive calibration signal that can be obtained by the calibration system. Sub-carriers of the receive calibration signal can be compared with sub-carriers of the reference signal to determine adjustments to transmission properties of antennas of the satellite.