The centrifugal channel device includes a channel body, a collecting unit and a waste liquid tank. The channel body includes a first surface and a second surface relatively disposed. The channel body includes a sample inlet, a sample channel, an isolation tank, a reagent inlet, a reagent channel and a mixing channel. The sample inlet is disposed on the first surface. The sample channel is connected to the sample inlet. The isolation tank is adjacently disposed and communicates with the sample channel. The reagent inlet is disposed on the first surface. The reagent channel is connected to the reagent inlet. One end of the mixing channel is connected with the sample channel and the reagent channel. The collecting unit has an opening and an overflow hole. The opening communicates with another end of the mixing channel. The waste liquid tank communicates with the opening of the collecting unit.