A positioning method for positioning two touch points of self-capacitive touch sensing devices is disclosed. The method includes: applying a first driving signals to the N rows of row sensors in sequence to determine the row sensors having the touch points; applying a second driving signals to the M columns of column sensors to determine the column sensor having touch points; applying a third driving signals to the row sensors or the column sensors; detecting a delay time period of the third driving signals within the row sensors or the column sensor so as to determine coordinates of the real touch points in accordance with the delay time period. In addition, a self-capacitive touch sensing device adopting the above method and a display device incorporating the above touch sensing device are also disclosed.