The present invention provides a method of compensating AMOLED IR Drop and a system. In the method of compensating AMOLED IR Drop, many times of iterated operations are performed to the power supply voltages and the driving currents of respective pixel driving circuits coupled in series on the same power supply line, and the adjustment and compensation are performed to the initial values Vdata1 to Vdatan of the data signal voltages for being inputted to respective pixel driving circuits according to the power supply voltages OVdd1 to OVddn of respective pixel driving circuits obtained with the last iterated operation of the calculation unit, and outputs the compensated data signal voltages Vdata1 to Vdatan corresponding to respective pixel driving circuits. The method can make that the driving currents flowing through respective pixels can be more uniform for solving the mura problem caused by IR Drop. The system of compensating AMOLED IR Drop can improve the brightness uniformity of an AMOLED display panel for solving the mura problem caused by IR Drop with setting the calculation unit, the storage unit, the compensation unit and the plurality of pixel driving circuits.