A tube connection assembly includes a first connector along a connection axis having a first engagement surface transverse to the connection axis, and a second connector connected to a tube, the second connector having a second engagement surface transverse to the connection axis. The first connector and the second connector are engagable together joining the first and second connectors in a coupled state, the first engagement surface and the second engagement surface spaced a predetermined distance when in the coupled state. A fastener is provided positionable in a locked state and an unlocked state, the fastener in the unlocked state being slidable along the tube, and the fastener in the locked state retaining the first engagement surface and the second engagement surface. The fastener may include a pair of opposing retaining surfaces spaced at least the predetermined distance, at least one of the retaining surfaces having a lead-in.