Invention Grant
- Patent Title: Cumulant microscopy
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Application No.: US14126712Application Date: 2012-03-31
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Publication No.: US09658442B2Publication Date: 2017-05-23
- Inventor: Stefan Geissbuehler , Claudio Dellagiacoma , Matthias Geissbuehler , Theo Lasser , Marcel Leutenegger
- Applicant: Stefan Geissbuehler , Claudio Dellagiacoma , Matthias Geissbuehler , Theo Lasser , Marcel Leutenegger
- Applicant Address: CH Lausanne
- Assignee: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
- Current Assignee: ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE (EPFL)
- Current Assignee Address: CH Lausanne
- Agency: Nixon & Vanderhye P.C.
- Priority: EP11169880 20110614
- International Application: PCT/IB2012/051581 WO 20120331
- International Announcement: WO2012/172440 WO 20121220
- Main IPC: H04N7/18
- IPC: H04N7/18 ; G02B21/36 ; G01N21/64 ; G02B21/16 ; G02B27/58

Abstract:
The invention describes a method and a microscopy system for imaging and analyzing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction of super-resolved environment-related parameter maps, such as molecular state lifetimes, concentration and brightness distributions of the emitter. In addition, such parameter maps can be used to compensate for the non-linear brightness and blinking response of higher-order cumulant images—used for example in Super-resolution Optical Fluctuation Imaging (SOFI)—to generate a balanced image contrast. Structures that otherwise would be masked by brighter regions in the conventional cumulant image become samples using spectral cross-cumulants.
Public/Granted literature
- US20140198198A1 CUMULANT MICROSCOPY Public/Granted day:2014-07-17
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