Adjustable allograft templates and methods of use are disclosed where a template for resecting a tissue region may generally comprise a template frame having a surface for contacting the tissue region and which defines an open area of tissue to be resected. A frame assembly along the template frame may be translatable relative to the template to adjust a size of the open area of tissue to be resected. One or more inner guidance slots may be defined along the frame such that the inner guidance slots are spaced relative to one another to define the tissue within the open area to be resected from a patient. Additionally, one or more outer guidance slots may also be defined along the frame and aligned adjacent to the inner guidance slots. The outer guidance slots may be spaced relative to one another to define the tissue to be resected from a donor.