Invention Grant
- Patent Title: Methods for reducing accumulation of lactate during culturing and method for producing polypeptide
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Application No.: US14114525Application Date: 2012-04-27
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Publication No.: US09670519B2Publication Date: 2017-06-06
- Inventor: Ruchika Srivastava , Sneha Lakshmandas Hemdev , Ankur Bhatnagar , Saravanan Desan , Anuj Goel , Harish Iyer , Vana Raja , Lavanya Rao
- Applicant: Ruchika Srivastava , Sneha Lakshmandas Hemdev , Ankur Bhatnagar , Saravanan Desan , Anuj Goel , Harish Iyer , Vana Raja , Lavanya Rao
- Applicant Address: IN Bangalore
- Assignee: Biocon Limited
- Current Assignee: Biocon Limited
- Current Assignee Address: IN Bangalore
- Agency: Schwegman Lundberg & Woessner, P.A.
- Priority: IN1486/CHE/2011 20110429
- International Application: PCT/IB2012/052108 WO 20120427
- International Announcement: WO2012/147048 WO 20121101
- Main IPC: C12P21/00
- IPC: C12P21/00 ; C07K16/28 ; C12N5/00
Abstract:
The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.
Public/Granted literature
- US20140051124A1 METHODS FOR REDUCING ACCUMULATION OF LACTATE DURING CULTURING AND METHOD FOR PRODUCING POLYPEPTIDE Public/Granted day:2014-02-20
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