The present invention provides a mutual capacitance one glass solution touch panel and a manufacture method thereof. A first through hole and a second through hole are formed at an isolation layer of the touch panel. A first sub electrode pattern of the touch panel is electrically connected to a fourth sub electrode pattern of the touch panel via the first through hole, and a third sub electrode pattern of the touch panel is electrically connected to a second sub electrode pattern of the touch panel via the second through hole as touch control test is performed. According to the aforesaid arrangement, the present invention is capable of reducing the wire resistance of the transmission signals in the electrode patterns to raise the touch control sensitivity.