Disclosed are a structure for improving electrical signal isolation in a semiconductor substrate and an associated method for the structure's fabrication. The structure includes a deep trench having sidewalls disposed in the semiconductor substrate. An isolation region may be formed along at least an upper portion of the sidewalls of the deep trench, and a metallic filler may be disposed in the deep trench. The isolation region may include a PN junction formed by one or more of ion implantation and annealing, deposition of highly doped polysilicon and out diffusion, and gas phase doping and annealing. In the alternative, the isolation region may be a dielectric isolation region formed by one or more of uniform dielectric deposition, partial dieletric deposition, and dielectric deposition by ionic reaction.