A separation assembly for separation of a fluid into first and second parts is disclosed. A container has a first end, a second end, and a sidewall extending therebetween defining an interior, the container defining a longitudinal axis between the first end and the second end. A separator body is disposed within the interior having a through-hole defined therethrough. The separator body includes a first part, and a second part interfaced with the first part, wherein the separator body is transitionable from a first position wherein the through-hole is provided in fluid-receiving alignment with the first end of the container, to a second position wherein the through-hole is provided substantially perpendicular to the longitudinal axis of the container. In the first position, a through-axis of the through-hole of the separator body is in a plane that is not parallel with a plane containing the longitudinal axis of the container.