A five-degree-of-freedom adjustment and positioning method and apparatus for assembly/measurement of rotor and stator of an aircraft engine; said method comprises adjusting a plane motion and a rotation of a tested piece through composite motion comprising five degrees of freedom: a 360° rotatory motion around a Z axis, a plane motion along an X axis and a plane motion along a Y axis, a rotatory motion around the X axis and a rotatory motion around the Y axis; said apparatus comprises: a clamping mechanism, a turning platform component, a translational platform component and a rotational platform component. The present invention designs a five-degree-of-freedom adjustment and positioning method and apparatus having properties of large load bearing, high precision and high stiffness, thus improving assembly efficiency and measurement accuracy of the aircraft engine.