Provided are an AMOLED pixel unit circuit, a driving method thereof and a display panel for realizing the threshold voltage compensation. The AMOLED pixel unit circuit includes: a light emitting module: a driving module configured to drive the light emitting module; a light emitting control module configured to control the light emitting module to emit light; a threshold compensation module configured to perform threshold voltage compensation for the driving module; a data voltage writing module configured to input a data voltage to the driving module; and an initialization module configured to initialize the threshold compensation module. The threshold voltage compensation is realized by modifying the pre-charging fashion to fixedly set the gate of the driving TFT to be at a data level lower than the high level such that the subthreshold saturation cutoff state is reached before the drain-source voltage becomes zero in the compensation stage.