A fluorescence detection system includes a light source for coupling with a mobile device and software, having machine readable instructions loadable into memory of the mobile device and executable by a processor of the mobile device, capable of: activating the light source in coordination with capturing one or more images using a camera of the mobile device; analyzing an image captured with the light source on with an image captured with the light source off, to detect a shift of intensity within a predetermined color range within the images; and identifying and authenticating objects by measuring the intensity shift within the images.