The disclosure discloses a method for interference cancellation of a data channel. The method includes that: bit-level channel recoding is performed on received transport block data, and then channel estimation is performed on the coded data and multiplexed antenna data of a data channel to obtain channel estimation data; data channel reconstruction is performed on the coded data with a Reconstruction Unit (RU) as a unit by virtue of the channel estimation data; and obtained reconstruction data is subtracted from the antenna data to implement interference cancellation. The disclosure further discloses a system for interference cancellation of a data channel.