Invention Grant
- Patent Title: Mutant Staphylococcus beta-glucuronidase enzymes with enhanced enzymatic activity
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Application No.: US15076134Application Date: 2016-03-21
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Publication No.: US09719075B2Publication Date: 2017-08-01
- Inventor: Lim Andrew Lee
- Applicant: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS
- Applicant Address: US SC Irmo
- Assignee: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS
- Current Assignee: INTEGRATED MICRO-CHROMATOGRAPHY SYSTEMS
- Current Assignee Address: US SC Irmo
- Agency: Nelson Mullins Riley & Scarborough LLP
- Agent Jill Gorny Sloper, Esq.
- Main IPC: C12N9/24
- IPC: C12N9/24 ; C12Q1/34
Abstract:
Mutated Staphylococcus sp. RLH1 β-glucuronidase enzymes with enhanced enzymatic activity and thermostability as compared to wild type enzyme are provided. The enzymes of the invention advantageously allow for accurate analysis of bodily samples for the presence of drugs in 30 minutes or less, as compared to the several hours needed using prior enzyme preparations. Methods of using the mutated enzymes for hydrolysis of glucuronide substrates, including opiates and benzodiazepines, are also provided.
Public/Granted literature
- US20160237415A1 MUTANT STAPHYLOCOCCUS BETA-GLUCURONIDASE ENZYMES WITH ENHANCED ENZYMATIC ACTIVITY Public/Granted day:2016-08-18
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