Invention Grant
- Patent Title: Methods for adding adapters to nucleic acids and compositions for practicing the same
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Application No.: US14566445Application Date: 2014-12-10
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Publication No.: US09719136B2Publication Date: 2017-08-01
- Inventor: Craig Betts , Andrew Alan Farmer
- Applicant: Clontech Laboratories, Inc.
- Applicant Address: US CA Mountain View
- Assignee: Takara Bio USA, Inc.
- Current Assignee: Takara Bio USA, Inc.
- Current Assignee Address: US CA Mountain View
- Agency: Bozicevic, Field & Francis LLP
- Agent Otto C. Guedelhoefer, IV; Bret E. Field
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C12N15/10

Abstract:
Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits.
Public/Granted literature
- US20150203906A1 METHODS FOR ADDING ADAPTERS TO NUCLEIC ACIDS AND COMPOSITIONS FOR PRACTICING THE SAME Public/Granted day:2015-07-23
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