An object of the present invention is to provide a honeycomb filter having low pressure loss and high collection efficiency. The honeycomb filter of the present invention comprises a ceramic honeycomb substrate in which a multitude of cells through which a fluid flows are disposed in parallel in a longitudinal direction and are separated by cell walls, each cell being sealed at an end section at either the fluid inlet side or the fluid outlet side, and a filter layer which, among the surfaces of the cell walls, is formed on the surface of the cell walls of those cells in which the end section at the fluid inlet side is open and the end section at the fluid outlet side is sealed by a sealing material, wherein the filter layer comprises spherical ceramic particles, and the average particle size of the spherical ceramic particles increases gradually from the fluid inlet side toward the fluid outlet side.