Invention Grant
- Patent Title: Protein tag, tagged protein, and protein purification method
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Application No.: US14390957Application Date: 2012-11-21
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Publication No.: US09751922B2Publication Date: 2017-09-05
- Inventor: Naoki Goshima , Eriko Fukuda , Masatoshi Mori
- Applicant: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
- Applicant Address: JP Tokyo
- Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
- Current Assignee: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
- Current Assignee Address: JP Tokyo
- Agency: Birch, Stewart, Kolasch & Birch, LLP
- Priority: JP2012-087214 20120406
- International Application: PCT/JP2012/080133 WO 20121121
- International Announcement: WO2013/150680 WO 20131010
- Main IPC: C12P21/04
- IPC: C12P21/04 ; C07K14/47 ; C12P21/02 ; C12N15/67

Abstract:
A technology which enables a high yield and convenient recovery of a protein and also enables a global protein purification is provided.A protein tag comprising an amino acid sequence of the full length or a part of MafG protein or an amino acid sequence in which amino acids serving as protease cleavage sites were inserted into the former amino acid sequence is provided. Since this protein tag can impart a high insolubility attributable to MafG protein to a protein to be tagged thereby insolubilizing the tagged protein, the tagged protein can be recovered into an insoluble fraction at a high yield.
Public/Granted literature
- US20150166619A1 PROTEIN TAG, TAGGED PROTEIN, AND PROTEIN PURIFICATION METHOD Public/Granted day:2015-06-18
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