A honeycomb structure including a honeycomb structure body, first plugging portions, and second plugging portions. The honeycomb structure body has a porous partition wall. The first plugging portions are disposed at an outflow side end face of inlet cells and an inflow side end face of outlet cells. The inlet cells are the predetermined cells of the honeycomb structure body. The outlet cells are the residual cells of the honeycomb structure body. The second plugging portions are not fired. The first plugging portions form a checkered pattern at the inflow side end face and the outflow side end face. The number of the second plugging portions is within 3% of the number of cell open ends where the first plugging portions are not formed, on both end faces of the honeycomb structure body.