Invention Grant
- Patent Title: Automated selection of microorganisms and identification using MALDI
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Application No.: US15383573Application Date: 2016-12-19
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Publication No.: US09753045B2Publication Date: 2017-09-05
- Inventor: Jetze Botma , Martijn Kleefstra , Tino Walter Van Der Zee
- Applicant: BD Kiestra B.V.
- Applicant Address: NL Drachten
- Assignee: BD KIESTRA B.V.
- Current Assignee: BD KIESTRA B.V.
- Current Assignee Address: NL Drachten
- Agency: Botos Churchill IP Law LLP
- Main IPC: C12M1/00
- IPC: C12M1/00 ; G01N35/00 ; C12M1/22 ; C12M1/36 ; G01N35/10 ; H01J49/16 ; G01N1/04

Abstract:
A method and apparatus for locating and selecting a colony of microorganisms on a culture dish and identifying microorganisms in said selected colony using MALDI. The method comprises the automated steps of locating and selecting a colony of microorganisms on a culture dish; obtaining a sample of said selected colony of microorganisms; depositing at least some of said sample of said selected colony of microorganisms on a target plate; and transferring said target plate with said sample in an apparatus for performing MALDI for identification of said sample of said selected colony of microorganisms. A sample of a colony of microorganisms is automatically deposited on a depositing spot such that the sample covers at most approximately half of said one of the depositing spots of the target plate. A suspension of a sample of microorganisms is automatically prepared by automatically picking the sample with a picking tool and submerging the picking tool with said sample in a suspension, after which the picking tool is vibrated in vertical sense only to release the sample from the picking tool.
Public/Granted literature
- US20170097368A1 AUTOMATED SELECTION OF MICROORGANISMS AND IDENTIFICATION USING MALDI Public/Granted day:2017-04-06
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