The present disclosure illustrates an acoustic echo cancellation method and system using the same. The acoustic echo cancellation method comprises the following steps. Firstly, a prior-knowledge matrix comprising a plurality of space vectors is built. Then, an initial filter vector is generated by the prior-knowledge matrix and an initial weighting vector. The weighting vector is updated based on the difference of the echo signal and the estimated signal in an iteration algorithm, and the coefficient of the filter vector is updated according to the updated weighting vector. An estimated signal is generated according to the updated filter vector and the original signal. Finally, the next echo signal is cancelled by the near-end estimated signal.